Melatonin’s Antioxidative Characteristic in Human Aging Retinal Pigment Epithelial Cells

In this study, the antioxidative effect of melatonin was investigated in aging retinal pigment epithelial cells vitro. The objective study is to explore administration pharmacological doses for prevention symptoms.Specific concentrations (20 µM, 40 µM and 80 µM) were used treat hydrogen peroxide-induced flow cytometry employed examine cell apoptosis. A specific probe utilized detect intracellular reactive oxygen species concentration apoptosis-associated proteins detected real-time polymerase chain reaction. Commercially available assay kits expression oxidative biomarkers, superoxide dismutase, malondialdehyde glutathione.In comparison normal cells, model had lower viability, higher apoptosis rates a more severe oxidation status. Melatonin increased viability while lowering stress. It influenced apoptosis-related as well stress indicators. Finally, treatment with able regulate proliferation, cells.The application may be novel strategy protect against age-related changes macular degeneration. has protective effects death. inhibits damage decreases rate.